| Author | Message | ||
     Juerg Senior Member Username: Juerg Post Number: 745 Registered: 04-2006 |
traditionally lactate is taken by the jugularis with a syruinge, but there is a much easier metghod as youy can see by this testing and we tried it with the same success so all coaches and horse trainers can do that pretty easy now with the lancet and a normal level 5 depth on the poker. Simple Lactate Measurement in Horses Using a Portable Lactate Analyzer with Lancet Skin Punctures under Field Conditions Mitsutoshi KOBAYASHI1) 1) Blood Horse Training Center Horse Clinic The following items were investigated to measure blood lactate in horses under field conditions using portable lactate analyzers recently developed for human athletes. 1) The precision of two types of portable lactate analyzer and the standard analyzer using the immobilized enzyme method, and correlation among the lactate concentrations measured by the three lactate analyzers. 2) The correlation between the lactate concentrations in peripheral blood collected by lancet puncture and jugular venous blood using Lactate-pro (L-pro). The study was performed in Thoroughbreds under training for races, using Accusport (AC) and L-pro as portable lactate analyzers and YSI1500 (YSI) for the standard analyzer using the immobilized enzyme method. The intra- and inter-assay coefficients of variation (CV) of L-pro was lower than AC at low and middle lactate concentrations. The precision of L-pro is better than AC when the blood lactate level is lower than 10 mmol/l, and hematocrit (Ht) is 57% or lower. The lactate concentration using L-pro in peripheral blood collected by lancet puncture through the neck skin was similar to the level in jugular venous blood. No infection or inflammatory symptom occurred after blood sampling and the examination is relatively safe. These findings suggest that blood lactate measurement using L-pro by lancet puncture through the neck skin under field conditions is simple and safe, requiring no blood sampling from the jugular vein, and it can be used for evaluation of aerobic capacity and intensity of exercise in daily submaximal training. | ||
| Joe_geiser Junior Member Username: Joe_geiser Post Number: 9 Registered: 07-2008 |
Juerg You can keep trying to sell the notion of getting enough blood from a lancet neck stick method if you want, but working with a sweating exercising horse has not worked for me even if you shave the area. It's so much easier to draw a cc of blood from the jug. Any horse person can do this with a bit of practice. After you draw the blood put a drop on a piece of waxed paper and touch the Lactate Pro strip to the drop of blood for immediate results. If one wants to use the "I'll test it later approach" as stated in the brochure you posted last night then inject the blood into a marked gray top tube. I think you need to then test for lactete within two hours. Of course the FaCT methodology is to get the results immediately as you will modify the exercises levels during the field test based on results. That's easy with the blood draw from the jug using the above approach. Regards Joe | ||
| Joe_geiser Member Username: Joe_geiser Post Number: 12 Registered: 07-2008 |
Here's still another approach to drawing blood. Go to http://www.polarequine.com Then read the opening story about the two German scientists Karsten Lütteken and Matthias Bojer. When you go to the next page you will see a photograph of some sort of tube with which they are drawing the blood I presume from a lancet stick. Although for me getting blood from the jug is easy, for some it may not be. Are you familiar with the tube shown in the picture? Of course the article is of general interest as it reinforces a growing interest of lactate testing for training horses. | ||
| Juerg Senior Member Username: Juerg Post Number: 747 Registered: 04-2006 |
Yes I am very familiar with this tube, as I did hundreds of test in the 1980 with this tube in St. Moritz. You need this if you use the few thousend dollar YSI lactate nalayzer, and it is fun to use it if it is very cold and very dusty and if the can do that on a horse on the neck , than the Lactate pro with teh lancet is small "cake" you have to watcgh with the tube , that there are no air bubbles in there, so the horse better keeps very quite. Than you have to "suck" the blood in a special syringe to have the absolue proper amount of blood and no airbubble, than you inject the blood through a special tube and a special membran and you before hand have a perfect mixture of some solution , as well you have to calibrate depending on the wind and wheather the equipment every time properly. Simple summary: You need a doctor and a lab technician to make a single sample. Now people know , why everybody from the traditional field was not amused as the Lactate pro was comming on the market. A second reason , why you take a capillary is , if you still use the Accu sport analyzer, as it is crucial to have a proper drop on the big test strip , otherwise you have a bad result. As well here we worked over many test with this device, as FaCT Canada was actually the company , who introduced the Accusport in North america over the former Company . Boerhinger Manheim , which than got bought up by La Roche Switzerland. This specific handling of blood over more than one step has a potential risk of wrong readings due to contamination , as well as a much higher risk of any contamination due to the handling of blood over more than one step. The last but not least question is: Lactate is a so called time sensitive test in hospitals , and lactate level ( values ) will change if not assessed immediatly , as well if not concerved porperly in the proper tubes. There is an ongoing discussion , how fast the values actually may change , and with the now accepted fact , that lactate can be transported in and out of cells with MCT proteins, there are many people asking the question , how active this MCT transporters still are in the blood and therefor any delay in sampling and assessing may be of a certain risk of changeing in values.. For FaCT users with our system , this concern is much smaller, as long we have always app. the same amount of time from te moment we take the blood to the moment of the readout. So the technique suggested with the paper by Joe is one of the possibilities as the steps will take always about the same time. I am waiting on some answers from a Vet. Dr. form our region a patinet of mine , with the result of the problem on too many sampling on a jugularis on a horse and will keep you updated on that one as information comes in. Cheers to all of you . Juerg | ||
| Joe_geiser Member Username: Joe_geiser Post Number: 13 Registered: 07-2008 |
Juerg, Thank you for your very comprehensive information. The tube idea was just a thought as an alternative. Yes, please update on the information from your Vet parient regarding too many jug samplings. Joe |